Yusrida Darwis's research while affiliated with Universiti Sains Malaysia and other places
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Publications (65)
Hibiscus sabdariffa L. (H.S.) plant and its calyces have received much attention from researchers because of their potential medicinal and nutritional values. Calyces are the major source of anthocyanin in this plant. Therefore, a well-developed, efficient, and accurate analytical method is needed to assure proper standardization and control the qu...
Lung cancer (LC) is the leading cause of cancer-related deaths, responsible for approximately 18.4% of all cancer mortalities in both sexes combined. The use of systemic therapeutics remains one of the primary treatments for LC. However, the therapeutic efficacy of these agents is limited due to their associated severe adverse effects, systemic tox...
Introduction: The self-emulsifying drug delivery system (SEDDS) has emerged as an effective pharmaceutical strategy for addressing the issue of poorly soluble drug bioavailability, specifically candidates belonging to BCS classes II and IV including azithromycin (AZM, log p = 4). Aside from oil, the main component in SEDDS is the surfactant, which...
Azithromycin (AZM) is a macrolide antibiotic used for the treatment of various bacterial infections. The drug is known to have low oral bioavailability (37%) which may be attributed to its relatively high molecular weight, low solubility, dissolution rate, and incomplete intestinal absorption. To overcome these drawbacks, liquid (L) and solid (S) s...
Introduction: Insulin is given by injection, because when administered orally, it would be destroyed by enzymes in the digestive system, hence only about 0.1% reaches blood circulation. The purpose of the present study was to use pH sensitive polyelectrolyte methyl methacrylate (MMA)/itaconic acid (IA) nanogels as carriers in an attempt to improve...
Curcuminoids have been used for the management of burns and wound healing in traditional Chinese medicine practices but the wide application of curcuminoids as a healing agent for wounds has always been a known problem due to their poor solubility, bioavailability, colour staining properties, as well as due to their intense photosensitivity and the...
Nanostructured lipid carriers (NLCs) loaded with lopinavir (LPV) were prepared by the high-shear homogenization method. The LPV-NLCs formulations were freeze-dried using trehalose as a cryoprotectant. In vitro release studies in simulated gastric fluid (pH 1.2) and simulated intestinal fluid (pH 6.8) showed a burst release. The optimized freeze-dri...
Verapamil is a calcium channel blocker and highly effective in the treatment of hypertension, angina pectoris, and other diseases. However, the drug has a low bioavailability of 20 to 35% due to the first pass effect. The main objective of this study was to develop hybrid verapamil-dextran nanostructured lipid carriers (HVD-NLCs) in an attempt to i...
Introduction: Colchicine is used for the treatment of gout, pseudo-gout, familial Mediterranean fever, and many other illnesses. Its oral administration is associated with poor bioavailability and severe gastrointestinal side effects. The drug is also known to have a low therapeutic index. Thus to overcome these drawbacks, the transdermal delivery...
Nanogels are polymeric nanoparticles that have similar characteristic to hydrogels, but have the size in nano range. The pH-sensitive nanogel have gained much interest in the field of pharmaceutical nanotechnology as they have potential to be used as nanocarriers in drug delivery system. The aim of the present study was to synthesize pH-sensitive p...
The objective of this work is to develop and validate a simple, rapid and specific reverse phase HPLC–UV method for the determination of azithromycin (AZM) in bulk, and self-emulsifying drug delivery system (SEDDs). The separation was done using Hypersil GOLD C-18 analytical column packed with deactivated silica (250 mm x 4.6 mm ID x 5 µm) kept at...
![Fig. 1: The chemical structure of colchicine [1]](profile/Reem-Abou-Assi/publication/318960228/figure/fig1/AS:525082087706624@1502200733283/The-chemical-structure-of-colchicine-1_Q320.jpg)
Objective: To develop and validate a stability-indicating reversed phase high-performance liquid chromatography (RP-HPLC) method for the determination of colchicine in bulk and ethosomal gel nano-formulation. Methods: The chromatographic conditions were optimized using stainless steel Hypersil Gold C-18 analytical column with the dimensions of 250...
Ethosomal systems are novel lipid vesicular carriers containing a relatively high percentage of ethanol. These nanocarriers are especially designed for the efficient delivery of therapeutic agents with different physicochemical properties into deep skin layers and across the skin. Ethosomes have undergone extensive research since they were invented...
Tuberculosis (TB) is the main infectious disease causing 1.8 million deaths worldwide every year and represents a principal cause of mortality resulting from a bacterial infection. The emergence of multidrug-resistant strains and lack of effective anti-TB drugs are threatening the future control of TB. The present multidrug regimen against TB needs...
Solid lipid nanoparticles of atovaquone (ATQ-SLN) were prepared by high shear homogenization method using tripalmitin, trilaurin, and Compritol 888 ATO as the lipid matrices and Phospholipon 90H, Tween 80, and poloxamer 188 as the surfactants. Optimization of the formulations was conducted using 6 sets of 2 4 full-factorial design based on four ind...
Nanogels have gained considerable importance as biomedical polymers, as polymeric biomaterials, and in drug delivery in the past two decades owing to their ability to absorb high contents of water and possess large number of functional groups, nanosize range, and swellable networks. In addition, nanogels offer great potential for utilization in the...
A new simple, sensitive and specific high performance liquid chromatography with UV detection (HPLC-UV) method was developed and validated for determination of Atovaquone in rabbit plasma. The method utilized 250 µl of plasma and Atovaquone was extracted from the plasma by using liquid-liquid extraction method. Buparvaquone was used as the internal...
Extensive research has been carried out for developing nanocarriers to overcome the major barriers preventing success in oral insulin therapy, which includes (a) identification of in vivo barriers, (b) incorporation of prerequisite characteristics into single nanocarrier, and (c) exclusion of the use of additional potentially toxic chemicals as enz...
Cinnamon leaf oil contains a high percentage of eugenol and has antimicrobial, antioxidant and antiinflammatory properties. However, the undiluted oil can cause irritation to the skin. Therefore, the aims of this study were to develop and evaluate cinnamon leaf oil nanocream using palm oil. Nanocream base was prepared using different ratios of oil,...
Traditional medical practitioners in Hawaii and Polynesia have used Morinda citrifolia L. (Noni) for centuries to cure or prevent varieties of illnesses. The popularity of M. citrifolia as a dietary supplement, a food functional ingredient, or as a natural health enhancer is increasing throughout the world. M. citrifolia contains phytochemicals tha...
A novel spectrophotometric method has been developed for the determination of cycloserine (CS) in capsules by derivatization with 4-dimethylaminocinnamaldehyde (DAC) to form CS-DAC derivative. The molar absorptivity of pure CS was calculated as 1.2 x 10(3) L/mole/cm at lambda(max) 288 nm and obeyed the Beer's law within 4-20 mu g/ml, whereas CS-DAC...
The present study was aimed to develop and validate a simple
reversed-phase high performance liquid chromatographic method for
determination of sulpiride in human plasma. The HPLC system was
operated at an excitation and emission wavelengths of 300 nm and 365
nm, respectively with the gain was set at 4 and sensitivity at medium.
The mobile pha...
The present study was aimed to develop and validate a simple reversed-phase high performance liquid chromatographic method for determination of sulpiride in human plasma. The HPLC system was operated at an excitation and emission wavelengths of 300 nm and 365 nm, respectively with the gain was set at 4 and sensitivity at medium. The mobile phase wa...
Purpose: To develop a new spectrophotometric method with improved sensitivity and at higher wavelength for the determination of p-aminosalicylic acid in tablets.Methods: Two simple and sensitive spectrophotometric methods (methods A and B) were developed using p-dimethylaminobenzaldehyde (DAB), and p-dimethylaminocinnamaldehyde (DAC) as derivatizin...
The delivery of drugs and bioactive compounds via the lymphatic system is complex and dependent on the physiological uniqueness of the system. The lymphatic route plays an important role in transporting extracellular fluid to maintain homeostasis and in transferring immune cells to injury sites, and is able to avoid first-pass metabolism, thus acti...
A simple and selective RP-HPLC-UV method with SPE was developed and validated for the quantification of cefotaxime in all-in-one total parenteral nutrition (AIO-TPN) admixtures. Chromatographic separation was achieved on a 5 pm particle size C18 DB column (250 x 4.6 mm id) using the mobile phase ammonium acetate (25 mM, pH 4.0)-50% acetonitrile in...
Lactulose is used as a triggering substance in a unique colon-specific delivery technology called CODES(TM). Colonic microflora degrades lactulose and forms short-chain fatty acids to activate the CODES(TM) system. However, lactulose has been reported to cause a Maillard-type reaction with substances containing primary or secondary amino groups tha...
Orthosiphon stamineus extracts contain three flavonoids (3'-hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin) as bioactive substances. Previous reported high performance liquid chromatography- ultraviolet (HPLC-UV) methods for the determination of these flavonoids have several disadvantages, including unsatisfactory separation times...
Strategy, Management and Health PolicyEnabling Technology, Genomics, ProteomicsPreclinical ResearchPreclinical Development Toxicology, Formulation Drug Delivery, PharmacokineticsClinical Development Phases I-III Regulatory, Quality, ManufacturingPostmarketing Phase IVNebulized corticosteroid drugs have several shortcomings due to their poor water s...
The local treatment of lung disorders such as asthma and chronic obstructive pulmonary disease via pulmonary drug delivery offers many advantages over oral or intravenous routes of administration. This is because direct deposition of a drug at the diseased site increases local drug concentrations, which improves the pulmonary receptor occupancy and...
A simple, specific and selective RP-HPLC-UV method with Solid Phase Extraction (SPE) was developed for the quantification of cefepime in Total Nutrient Admixtures (TNAs). Chromatographic separation was achieved on C18 column using a mobile phase consisting of a mixture of ammonium acetate, acetonitrile and methanol. The flow rate was set to 0.9 mL...
The aim of this study was to investigate the potential of a polyethylene glycol-phos- phatidylethanolamine conjugate (PEG 2000-DSPE) to solubilize budesonide (BUD) for pulmonary delivery. The BUD-strictly stabilized phospholipid nanomicelles (SSMs) were prepared using the coprecipitation and reconstitution method and the physicochemical characteris...
A new, sensitive and specific isocratic reverse phase-high performance liquid chromatography (RP-HPLC) method with fluorescence detection was developed and validated for the determination of sumatriptan in rabbit plasma using sulpiride as an internal standard (IS). Sumatriptan was extracted from plasma by a liquid-liquid extraction with a mixture o...
A new sensitive and specific isocratic RP-HPLC-UV method was developed and validated for the determination of ondansetron in rabbit plasma using risperidone as an internal standard (IS). The sample preparation involved a simple deprotenization procedure with a mixture of 1 mL of acetonitrile and 50 mu L of 10% w/v zinc sulfate. Analysis was perform...
The aim of this study was to formulate cost effective taste-masked orally disintegrating tablets of ondansetron, a bitter drug using different superdisintegrants by a wet granulation technique. Microcrystalline cellulose (Avicel) as a diluent and disintegrant in addition to aspartame as a sweetener were used in all formulations. The prepared tablet...
Inhaled corticosteroids provide unique systems for local treatment of asthma or chronic obstructive pulmonary disease. However, the use of poorly soluble drugs for nebulization has been inadequate, and many patients rely on large doses to achieve optimal control of their disease. Theoretically, nanotechnology with a sustained-release formulation ma...
The aims of the present research were to mask the intensely bitter taste of sumatriptan succinate and to formulate orally disintegrating tablets (ODTs) of the taste masked drug. Taste masking was performed by coating sumatriptan succinate with Eudragit EPO using spray drying technique. The resultant microspheres were evaluated for thermal analysis,...
A simple yet sensitive high performance liquid chromatography (HPLC) method was developed and validated for the determination of aerodynamic characteristics of the emitted dose of beclomethasone dipropionate from Beclate-50 Inhaler®. The mobile phase consisted of a mixture of acetonitrile and 10 mM ammonium acetate (70:30% v/v) adjusted to pH 5 wit...
A sensitive and rapid high performance liquid chromatography method was developed and validated for the determination of aerodynamic characteristics of the emitted dose of budesonide from different inhaler dosage forms. The mobile phase consisted of a mixture of acetonitrile and 10 mM ammonium acetate (63:37% v/v) adjusted to pH5 with orthophosphor...
Rifampicin-loaded nanoparticles were prepared using two different molecular weights of poly-(ethylene oxide)-block-distearoyl phosphatidyl-ethanolamine (mPEG2000-DSPE and mPEG5000-DSPE) polymers. Particle sizes of all formulations studied were in the range of 162-395 nm. The entrapment efficiency (EE) was not affected by the copolymer's molecular w...
The self-microemulsifying drug delivery system (SMEDDS) was employed to improve the bioavailability of sulpiride, a drug which is poorly soluble. The mean droplet size and emulsification time of the test formulation used for in vivo study were 9.27 +/- 2.02 nm and 87 +/- 5 s, respectively. When compared with Reference (Dogmatil (R)), the test formu...
The aim of this study was to prepare insulin-loaded poly(lactic acid)-polyethylene glycol microspheres that could control insulin release at least for 1 week and evaluate their in vivo performance in a streptozotocin-induced diabetic rat model.
The microspheres were prepared using a water-in-oil-in-water double emulsion solvent evaporation techniqu...
An isocratic RP-HPLC-UV method for analysis of sumatriptan succinate in pharmaceutical dosage forms has been developed and validated. Best separation was achieved on a Thermo Hypersil C4 column (250 mm X 4.6 mm, 5 mu m) using a mobile phase of 20 mM potassium dihydrogen phosphate adjusted to pH 4.0 with orthophosphoric acid and acetonitrile (65: 35...
A new, simple, rapid, sensitive and specific isocratic RP–LC–UV method was developed and validated for the determination of
ondansetron in pharmaceutical dosage forms of orally disintegrating tablets, oral solution and injection. The LC separation
was achieved on a Hypersil C4 column (250×4.6mm, 5μm) using a mobile phase of 50mM potassium dihydroge...
Angiogenesis is essential for the growth and metastasis of most solid malignancies. Accordingly, tumor angiogenesis is an important pharmacological target for cancer prevention and treatment. This study was conducted to study antiangiogenic activity of some tropical plants. Seven plants were extracted successively with n-hexane and methanol to prep...
Both cytotoxic and apoptotic properties of the stem bark extract of Sandoricum koetjape were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro-5- sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on three breast cancer cell lines and one normal cell line; MCF-7, MDA-MB-231, T47D a...
Cytotoxic and anti-angiogenic properties of n-hexane extract of Sandoricum koetjape stem bark were investigated in vitro. 2,3-bis-(2-methoxy-4- nitro- 5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on Human Umbilical Vein Endothelial Cell (HUVEC) and both colon cancer and nor...
The leaf material of lemon balm (Melissa officinalis L.) was extracted with 450 ml/l aqueous ethanol by medium pressure liquid–solid extraction. The total phenolic content of the extract was estimated as gallic acid equivalents by Folin–Ciocalteu reagent method and a qualitative–quantitative compositional analysis was carried out using high perform...
The aim of the present work was to investigate the preparation of microspheres as potential drug carriers for proteins, intended for controlled release formulation. The hydrophilic bovine serum albumin was chosen as a model protein to be encapsulated within poly(D,L-lactide-co-glycolide) (50:50) microspheres using a w/o/w double emulsion solvent ev...
A simple, sensitive high performance liquid chromatographic method with UV detection was developed and validated for determination
of insulin in rat plasma, using methyl paraben as an internal standard. Insulin was extracted from plasma by a liquid–liquid
extraction with a mixture of dichloromethane and n-hexane (1:1, v/v) followed by an acidic bac...
The potential of using poly-(ethylene oxide)-block-distearoyl phosphatidyl-ethanolamine (mPEG-DSPE) polymer to prepare BDP-loaded micelles with high entrapment efficiency and mass median aerodynamic diameter of less than 5 microm demonstrating sustained release properties was evaluated. The result showed that lyophilized BDP-loaded polymeric micell...
Citations
... The main H. sabdariffa L. classes of bioactive compounds in the context of therapeutic importance are polysaccharides, organic acids, flavonoids, and anthocyanins, which provide a high antioxidant capacity [4] and free radical scavenging activity [3]. Several studies revealed the therapeutic properties of H. sabdariffa L. extracts in which the major components are anthocyanins such as delphinidin-3-O sambubioside and cyanidin-3-O sambubioside [5,6]. Other flavonoids found in this extract are gossypetine, hibiscetin, and their respective glycosides, as well as protocatechuic acid, eugenol, and sterols like β-sitoesterol and ergoesterol [3]. ...
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... However, attachment of macrophages to the flat surfaces or minor axes of rectangular, elliptical disks, and oblate ellipsoids, are failed to engulf even after 2 h. Additionally, the worm-like particles had a considerably lower rate of phagocytosis clearance than the spherical particles because of their reduced bending area [202]. Therefore, in order to prolong lipid-based nanocarriers' pulmonary retention and delivery periods, particle-engineering technologies are employed to embed these NPs in MPs of different morphologies. ...
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... AZ is soluble at saliva pH; however, it is practically insoluble in water, causing the drug to solubilize in the mouth and exposure to taste buds, thus feeling bitter (2). Further, the potential of AZ to decompose in an acidic medium is high (3). A serious problem in the completion of the treatment, however, in sensitive patients as well as children is the obnoxious bitter taste, as a large number of people could not tolerate the drugs' bitter taste and may vomit out, leading to mental stress, grimace, and suboptimal therapeutic value (4). ...
... The amide I band shifted to 1659.64 cm − 1 in the vesicles due to ion cross-linking of the INS amide group with NPs, and the amide II band was also observed in NPs with less intensity at the same point in 1384.16 cm − 1 , which can be explained by the encapsulation of INS in the NPs [64,65]. ...
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... Then, the MTT assay was performed on L929 fibroblast cells cultivated in the growth medium diluted with the extracted leachates from hydrogels. The percentage of cell viability was calculated according to the following equation: [37]. ...
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... activity of the drug. (Ikeuchi-Takahashi et al., 2016;Khan et al., 2019;Sabapati et al., 2019) Therefore, the CU1-loaded SLN was prepared. A large number of single-factor experiments in the early stage were performed to preliminarily screen the dosage of drugs and excipients in the formula. ...
... It has been reported that the oral bioavailability and half-life of Ivabradine can be increased by adding it into polymeric nanoparticles [15]. Another study also reported a significant increase in the uptake of verapamil by the combination of verapamil and dextran in nanoliposome [63]. In addition, a novel biocomposite polymeric nanofiber for sublingual delivery of nicorandil can reduce mucosal ulceration and improve drug bioavailability successfully [16]. ...
... Nonetheless, dermatitis, allergic reaction, and skin irritation are among the transethosomes disadvantages.57 Abdulbaqi et al.58 delivered colchicine transdermally through three different formulations of transethosomal gels made with Phospholipon 90G® (PL90G), absolute ethanol, Tween 20®, sodium stearate, and triethanolamine via the cold method. The final gel formulation was formed by incorporating colchicine-loaded transethosomes in a pre-prepared gel with a ratio of 4:1 by using a stirrer and then manual mixing. ...
... Robustness A suitable HPLC method must have an adequate capacity to remain unaffected by minor variations in the method parameters. Thus, robustness studies are essential because the analyst may need to change one or more of the method's chemicals and/or instrumental parameters, and this can only be done if the changes do not affect the method's accuracy when compared to the previously optimized results [37,38]. Unfortunately, robustness studies were not performed in many of the developed HPLC methods for anthocyanin quantification [24,25,26,27,28]. ...
... The NGs were able to encapsulate and release the anionic dye new coccine. NGs of size about 250 nm and a good size distribution were obtained by copolymerization of methyl methacrylate (MMA) and itaconic acid (IA) in the presence of the ethylene glycol dimethacrylate (EGDMA) crosslinker [82]. In vitro experiments on Culture of Caco-2 cells and in vivo studies on female Sprague Dawly rats did not show any toxicity. ...
